UV Plate – Suzhou Calco Technology Co.,Ltd.

Characteristics:

96/384-well UV plate can let 80% , 260/280nm wavelength of UV light through its flat bottom interface, using this characteristic to quantify the content and purity of DNA and Protein.

A typical 96-hole transparent plate is made of PS、PC or PET, when the light source passes through a 260nm filter and the filtered light beam irradiates the interface of PS、PC or PET, the short wavelength ultraviolet light is absorbed by the medium and can not pass through the interface, so it can not be used as a UV plate.

UV Plate quantifies the amount of DNA and Protein in a sample:
When short-wavelength ultraviolet light at 260 nm strikes a DNA sample, it is absorbed by the DNA molecule. The penetration of ultraviolet light decreases, and the Optical Density measured by the opposite photometer rises in contrast, by measuring the value of the optical density, the software program can calculate the actual DNA content. When a Protein sample is exposed to 280 nm short-wavelength ultraviolet light, it is absorbed by the Protein molecule. The transmission of ultraviolet light decreases, and the Optical Density measured by the opposite photometer increases in contrast, by measuring the value of the optical density, the software program can calculate the actual content of DNA.

Experimental data： 260nm-280nm wavelength stability

Wavelength	OD	Wavelength	OD	Wavelength	OD	Wavelength	OD
		240	0.1189	282	0.0621	324	0.0509
200	2.3751	242	0.1124	284	0.0612	326	0.0507
202	2.355	244	0.1066	286	0.0604	328	0.0504
204	2.2778	246	0.1017	288	0.0596	330	0.05
206	2.0953	248	0.0981	290	0.0588	332	0.0496
208	1.9	250	0.0945	292	0.058	334	0.0493
210	1.6379	252	0.0911	294	0.0573	336	0.0489
212	1.3861	254	0.0874	296	0.0567	338	0.0486
214	1.1402	256	0.084	298	0.0562	340	0.0481
216	0.9283	258	0.0806	300	0.0558
218	0.7418	260	0.0788	302	0.0552
220	0.5968	262	0.0774	304	0.0547
222	0.4822	264	0.0755	306	0.0542
224	0.3858	266	0.0733	308	0.0537
226	0.2915	268	0.0713	310	0.0534
228	0.2423	270	0.0696	312	0.053
230	0.2112	272	0.0683	314	0.0527
232	0.1856	274	0.067	316	0.0522
234	0.1619	276	0.0657	318	0.0519
236	0.1424	278	0.0643	320	0.0516
238	0.1298	280	0.0632	322	0.0512